Identification of Thai isolates assigned to the genus <i>Gluconobacter</i> based on 16S-23S rDNA ITS restriction analysis
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Differential Identification of Flavobacterium Species by Sequence Analysis of Genus-Specific Hypervariable 16S-23S rDNA Intergenic Spacer Target
The aim of the current study was to develop a molecular system for differential identification of various fish diseases caused by Flavobacterium species. The system uses the hypervariability of the 16S-23S rDNA Intergenic spacer region (ISR) to develop PCR-based sequence analysis assay. For this purpose, eight different 16S-23S rDNA ISR sequences of six different Flavobacterium species were ali...
متن کاملInvestigation of the phylogenetic relationships within the genus Bartonella based on comparative sequence analysis of the rnpB gene, 16S rDNA and 23S rDNA.
A variety of genes and analytical methods have been applied to the study of phylogenetic relationships within the genus Bartonella, but so far the results have been inconsistent. While previous studies analysed single protein-encoding genes, we have analysed an alignment containing the sequences of three important phylogenetic markers, RNase P RNA, 16S rRNA and 23S rRNA, merged by catenation, t...
متن کاملJ. Gen. Appl. Microbiol., 53, 259–264 (2007)
duced in acetic acid bacteria with a single species, Asaia bogorensis Yamada et al. 2000 (Yamada et al., 2000). Since the second and the third species were additionally described as Asaia siamensis Katsura et al. 2001 and Asaia krungthepensis Yukphan et al. 2004, three species were described in total (Katsura et al., 2001; Yukphan et al., 2004). Strains of the three Asaia species show common ph...
متن کاملNew approaches to typing and identification of bacteria using the 16S-23S rDNA spacer region.
Medical microbiology is extremely reliant on the culture of bacteria from clinical specimens and their subsequent identification by biochemical and phenotypic characteristics for the diagnosis of disease. Following determination of the structure of DNA by Watson & Crick (1953), studies in bacteriology have seen a major shift from functional to molecular techniques for identifying bacteria (Town...
متن کاملIdentification of acetic acid bacteria by RFLP of PCR-amplified 16S rDNA and 16S-23S rDNA intergenic spacer.
DNA corresponding to 16S rDNA and the 165-23S rDNA intergenic spacer (ITS) from 22 reference strains of acetic acid bacteria, representing the diversity of the family Acetobacteraceae, and 24 indigenous acetic acid bacteria isolated from wine fermentations were analysed by PCR-RFLP. Frateuria aurantia LMG 1558T and Escherichia coli ATCC 11775T were included as outgroups. PCR-amplified products ...
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ژورنال
عنوان ژورنال: The Journal of General and Applied Microbiology
سال: 2007
ISSN: 0022-1260,1349-8037
DOI: 10.2323/jgam.53.133